Free cytosolic NAD+/NADH ratio

Range ~120 unitless
Organism Rat Rattus norvegicus
Reference Obrosova IG, Stevens MJ, Effect of dietary taurine supplementation on GSH and NAD(P)-redox status, lipid peroxidation, and energy metabolism in diabetic precataractous lens. Invest Ophthalmol Vis Sci. 1999 Mar40(3):680-8. p.684 fig 3APubMed ID10067971
Method "According to classic reports from the Krebs' laboratory [refs 35,36] and other studies, [ref 37] direct measurement of NAD, NADH, NADP, and NADPH is not informative in terms of control of metabolism because they do not provide information on compartmentalization of nicotinamide nucleotides between cytosol and mitochondria and because they do not separate free from protein-bound forms (only free fractions determine direction and free-energy changes of dehydrogenase reactions). The same studies proposed an alternative approach, implying assessment of free NAD(P)+/NAD(P)H ratios in the cytoplasm and mitochondria by measuring the ratios of the concentrations of the oxidized and reduced metabolites of suitable NAD(P)-linked dehydrogenase systems." Value extracted visually from fig.3A.
Comments "Free cytosolic NAD+/NADH ratio (Fig. 3A) was markedly reduced in diabetic rats versus control rats (P < 0.01). This reduction was prevented in diabetic rats fed the 5% taurine supplemented diet (P < 0.01 versus untreated diabetic rats)."
Entered by Uri M
ID 108110