in cytosol 100-2,000: in mitochondria 5-10 unitless
|Rat Rattus norvegicus
|Bekers KM, Heijnen JJ1, van Gulik WM. Determination of the in vivo NAD/NADH ratio in S. cerevisiae under anaerobic conditions using alcohol dehydrogenase as sensor reaction. Yeast. 2015 Jun 9. doi: 10.1002/yea.3078. p.542 left column 2nd paragraphPubMed ID26059529
|Williamson DH, Lund P, Krebs HA. The redox state of free nicotinamide-adenine dinucleotide in the cytoplasm and mitochondria of rat liver. Biochem J. 1967 May103(2):514-27.PubMed ID4291787
|P.542 left column 2nd paragraph: "The first drawback of whole cell measurements in eukaryotic cells is that only average values are obtained, and concentration differences between different cellular compartments cannot be observed. It is known from work with rat liver cells that the free NAD/NADH ratio can be very different in different cellular compartments, i.e. 100-2000 in the cytosol and 5-10 in mitochondria (primary source)."