Cytosolic NADH/NAD+ ratio in the dark

Range 0.0007 at pH 7.2: 0.0017 at pH 7.6 unitless
Organism Spinach Spinacia oleracea
Reference Heineke D, Riens B, Grosse H, Hoferichter P, Peter U, Flügge UI, Heldt HW. Redox Transfer across the Inner Chloroplast Envelope Membrane. Plant Physiol. 1991 Apr95(4):1131-1137 p.1134 left column bottom paragraphPubMed ID16668101
Method "Spinach plants were grown in a climatized chamber with a 9 h light/15 h dark cycle and leaves were stopped in liquid N2 after 8.5 h light or 2 h dark."
Comments "From the data of Table IV - link and assuming a pH of 7.2, the cytosolic NADH to NAD ratio in the light is calculated as 0.5×10^-3 which in the presence of 0.6 mM NAD corresponds to an NADH concentration of 0.3 µM. At an assumed cytosolic pH of 7.6, a NADH to NAD ratio of 1.1×10^-3 and a NADH concentration of 0.7 µM is evaluated. For leaves kept in darkness very similar values are found, the NADH to NAD ratio amounting to 0.7×10^-3 (pH 7.2) and 1.7×10^-3 (pH 7.6) corresponding to a NADH concentration of 0.5 and 1.2 µM, respectively."
Entered by Uri M
ID 108118