Decrease in velocity of the reaction when isocitrate replaces homoisocitrate as substrate

Value 216 Fold
Organism Budding yeast Saccharomyces cerevisiae
Reference Lin Y, Alguindigue SS, Volkman J, Nicholas KM, West AH, Cook PF. Complete kinetic mechanism of homoisocitrate dehydrogenase from Saccharomyces cerevisiae. Biochemistry. 2007 Jan 2346(3):890-8.PubMed ID17223711
Method Determination of the Mg-HIc Dissociation Constant by 1H NMR
Comments In contrast to HIc, the uncomplexed form of isocitrate and Mg2+ bind to the enzyme, unlike the situation with MgHIc.
Entered by Uri M
ID 102568