Keq for complete kinetic mechanism of homoisocitrate dehydrogenase

Value 0.45 M
Organism Budding yeast Saccharomyces cerevisiae
Reference Lin Y, Alguindigue SS, Volkman J, Nicholas KM, West AH, Cook PF. Complete kinetic mechanism of homoisocitrate dehydrogenase from Saccharomyces cerevisiae. Biochemistry. 2007 Jan 2346(3):890-8.PubMed ID17223711
Method The Keq for the overall reaction measured directly using the change in NADH as a probe, calculated using fixed concentrations of the other reactants and 2.3 mM a-Ka (see eq. 12)
Comments The Keq calculated from the Haldane relationship using the kinetic constants given in Table 1 and eq. 13 is 0.68 ± 0.03 M.
Entered by Uri M
ID 102566