Increse in weight after 7 hours of growth on Pi depleted medium

Value >=3 Fold
Organism Budding yeast Saccharomyces cerevisiae
Reference Vagabov VM, Trilisenko LV, Kulaev IS. Dependence of inorganic polyphosphate chain length on the orthophosphate content in the culture medium of the yeast Saccharomyces cerevisiae. Biochemistry (Mosc). 2000 Mar65(3):349-54. Fig. 1(a)PubMed ID10739478
Method The yeast is first cultivated for 4 h in a complete Rider medium containing 9 mM Pi and 111 mM glucose as a carbon source.Then the cells are placed on the same medium except depleted of Pi under sterile conditions and the culture is grown during 7 h. To determine dry weight, aliquots of cell suspensions were applied on filters preliminary brought to a constant weight by drying under vacuum at 85°C and dried to a constant weight under the same conditions.
Comments Almost complete polyP utilization of absolutely all fractions is seen within 7 h of phosphate starvation.
Entered by Uri M
ID 103443