||Budding yeast Saccharomyces cerevisiae
||Vagabov VM, Trilisenko LV, Kulaev IS. Dependence of inorganic polyphosphate chain length on the orthophosphate content in the culture medium of the yeast Saccharomyces cerevisiae. Biochemistry (Mosc). 2000 Mar65(3):349-54. Fig. 1(a)PubMed ID10739478
||The yeast is first cultivated for 4 h in a complete Rider medium containing 9 mM Pi and 111 mM glucose as a carbon source.Then the cells are placed on the same medium except depleted of Pi under sterile conditions and the culture is grown during 7 h. To determine dry weight, aliquots of cell suspensions were applied on filters preliminary brought to a constant weight by drying under vacuum at 85°C and dried to a constant weight under the same conditions.
||Almost complete polyP utilization of absolutely all fractions is seen within 7 h of phosphate starvation.