Increase in length of polyphosphate chain in PolyP2 fraction during 20 fold decrease of weight following Phosphorus depletion

Value 2 Fold
Organism Budding yeast Saccharomyces cerevisiae
Reference Vagabov VM, Trilisenko LV, Kulaev IS. Dependence of inorganic polyphosphate chain length on the orthophosphate content in the culture medium of the yeast Saccharomyces cerevisiae. Biochemistry (Mosc). 2000 Mar65(3):349-54.PubMed ID10739478
Method The yeast is first cultivated for 4 h in a complete Rider medium containing 9 mM Pi and 111 mM glucose as a carbon source. Then the cells are placed on the same medium except depleted of Pi under sterile conditions and the culture is grown during 7 h. Further, the cells are again placed on fresh complete medium, where the culture is growing for 2 and 4 h. The degree of polymerization (n) of polyP fractions was estimated from the areas of 31P-NMR-spectral peaks.
Comments This is the salt-soluble fraction (polyP2). The length of the chains increased to n=40-45 Pi units
Entered by Uri M
ID 103448