Decrease in RNA polymerase III elongation rate at 0 degrees celsius

Value 30 Fold
Organism Budding yeast Saccharomyces cerevisiae
Reference Matsuzaki H, Kassavetis GA, Geiduschek EP. Analysis of RNA chain elongation and termination by Saccharomyces cerevisiae RNA polymerase III. J Mol Biol. 1994 Jan 28235(4):1173-92.PubMed ID8308883
Method In vitro transcription and transcript release. Autoradiograms were scanned with a laser densitometer and analyzed with the aid of a software package developed by AMBIS (san diego)
Comments RNA chain elongation through assembled transcription complexes was uneven but relatively rapid: at 20 degrees C with 1 mM NTPs, the fastest RNA chains elongated at an average rate of 29 nucleotides (nt)/second, and the median RNA chains elongated at 21 to 22 nt/second on average. At 0 degrees celsius rate decreased 30-fold
Entered by Uri M
ID 103658