| Value |
180
ms
Range: ±10 [docking (80 ms), transport (5-20 ms) and release (80 ms)] ms
|
| Organism |
Mouse Mus musculus |
| Reference |
Grünwald D, Singer RH. In vivo imaging of labelled endogenous ß-actin mRNA during nucleocytoplasmic transport. Nature. 2010 Sep 30 467(7315):604-7. doi: 10.1038/nature09438. abstract & p.605 left column bottom paragraphPubMed ID20844488
|
| Method |
Abstract:"Here [investigators] developed a super-registration approach using fluorescence microscopy that can overcome the current limitations of co-localization by means of measuring intermolecular distances of chromatically different fluorescent molecules with nanometre precision. With this method [they] achieve 20-ms time-precision and at least 26-nm spatial precision, enabling the capture of highly transient interactions in living cells." |
| Comments |
Abstract:"Using this approach [investigators] were able to spatially resolve the kinetics of mRNA transport in mammalian cells and present a three-step model consisting of docking (80 ms), transport (5-20 ms) and release (80 ms), totalling 180 ± 10 ms." p.605 left column bottom paragraph:"The kinetic analyses gave a total transport time of ~180 ms (Fig. 2). The binding site analysis combined with the channel translocation time argues for a three-step transport mechanism that involves nucleoplasmic docking (~80 ms), a fast translocation through the central channel (5–20 ms) and a cytoplasmic release step (~80 ms) (Fig. 4). The symmetry in the nuclear and cytoplasmic binding frequencies argues for similar kinetics on both sides of the pore." Immortalized mouse embryo fibroblast cells (MEFs) |
| Entered by |
Uri M |
| ID |
111844 |