NPC (nuclear pore complex) density on nuclear envelope in MDCK

Value 7 pores/µm^2 Range: Table - link pores/µm^2
Organism Mammalian tissue culture cell
Reference Mazzanti M, Bustamante JO, Oberleithner H. 2001. Electrical dimension of the nuclear envelope. Physiol. Rev. 81: 1–19 p.5 table 1 & p.11 right column top paragraphPubMed ID11152752
Primary Source 114) Oberleithner H, Brinckmann E, Schwab A, Krohne G. Imaging nuclear pores of aldosterone sensitive kidney cells by atomic force microscopy. Proc Natl Acad Sci USA 91: 9784–9788, 1994. (116) Oberleithner H1, Schneider S, Bustamante JO. Atomic force microscopy visualizes ATP-dependent dissociation of multimeric TATA-binding protein before translocation into the cell nucleus. Pflu¨ gers Arch 432: 839–844, 1996.PubMed ID7937891, 8772134
Comments "Direct comparison of the data for the different preparations reveals that GNE (total nuclear envelope conductance) varied by at least a factor of 10. At first sight this is not very surprising since NPC density per unit area can vary among different species to a similar extent (refs 104, 144, 145). However, variations in GNE occurred virtually independent of NPC density. The NE of Chironomus salivary gland, for example, showed a similar GNE per cm^2 (40 pores/µm^2) (refs 71, 125) compared with GNE of cultured kidney cells (7 pores/µm^2) (primary sources)." See notes beneath table. MDCK=Madin Darby canine kidney
Entered by Uri M
ID 110698