Number of NPC (nuclear pore complexes) in a G1 cell nucleus

Range ~90 NPCs/nucleus
Organism Budding yeast Saccharomyces cerevisiae
Reference Oeffinger M, Zenklusen D. To the pore and through the pore: a story of mRNA export kinetics. Biochim Biophys Acta. 2012 Jun1819(6):494-506. doi: 10.1016/j.bbagrm.2012.02.011. p.498 left column 2nd paragraphPubMed ID22387213
Primary Source [57] M. Winey, D. Yarar, T. Giddings, D. Mastronarde, Nuclear pore complex number and distribution throughout the Saccharomyces cerevisiae cell cycle by three dimensional reconstruction from electron micrographs of nuclear envelopes, Mol. Biol. Cell 8 (1997) 2119–2132.PubMed ID9362057
Method Cryo-electron microscopy (cryo-EM), or electron cryomicroscopy,
Comments P.498 left column 2nd paragraph: "Reaching the nuclear periphery is the first step on the way out of the nucleus. Once mRNPs have reached the nuclear periphery, they have to encounter an NPC, interact with its structure and get access to the pore. Thus encountering a nuclear pore is the next step. The surface of the inner nuclear membrane is densely packed with nuclear pore complexes. Cyro-EM studies in yeast have shown that a G1 cell nucleus contains about 90 NPCs [primary source]."
Entered by Uri M
ID 110670