| Range |
Table - link
|
| Organism |
Rat Rattus norvegicus |
| Reference |
Soboll S, Scholz R, Heldt HW. Subcellular metabolite concentrations. Dependence of mitochondrial and cytosolic ATP systems on the metabolic state of perfused rat liver. Eur J Biochem. 1978 Jun 15 87(2):377-90. p.386 table 8PubMed ID668699
|
| Method |
“From marker enzyme activities and metabolite
contents in the unfractionated homogenate and in
the different fractions the mitochondrial and cytosolic
metabolite contents (nmol/mg protein) were calculated
by extrapolating the data to fractions of pure mitochondria
and pure cytosol according to Elbers et al.
[ref 5]. The subcellular metabolite contents were converted
into concentrations assuming a water content of 0.8 and 3.8 µl/mg protein in the mitochondrial and
cytosolic compartment, respectively, based on considerations
previously discussed by Soboll et al. [ref 1].” |
| Comments |
"As shown in Table 8, the cytosolic and mitochondrial concentrations of inorganic phosphate are similar and appear to be unaffected by the nutritional state of the donor animal or the metabolic condition of the liver. The data suggest that changes in the phosphorylation potentials are reflected entirely by changes in the ATP/ADP ratios under all metabolic conditions." For experimental conditions and metabolic states see p.381 table 1 link |
| Entered by |
Uri M |
| ID |
111358 |