Contents of adenine nucleotides in liver tissue

Range Table - link
Organism Rat Rattus norvegicus
Reference Soboll S, Scholz R, Heldt HW. Subcellular metabolite concentrations. Dependence of mitochondrial and cytosolic ATP systems on the metabolic state of perfused rat liver. Eur J Biochem. 1978 Jun 15 87(2):377-90. p.383 table 4PubMed ID668699
Method “From marker enzyme activities and metabolite contents in the unfractionated homogenate and in the different fractions the mitochondrial and cytosolic metabolite contents (nmol/mg protein) were calculated by extrapolating the data to fractions of pure mitochondria and pure cytosol according to Elbers et al. [ref 5]. The subcellular metabolite contents were converted into concentrations assuming a water content of 0.8 and 3.8 µl/mg protein in the mitochondrial and cytosolic compartment, respectively, based on considerations previously discussed by Soboll et al. [ref 1].”
Comments "When the freeze-dried liver tissue was separated into mitochondrial and cytosolic fractions, only 20 - 27 of the overall tissue content of adenine nucleotides were found in the mitochondria (Table 4, right column). The observation of a non-uniform distribution of adenine nucleotides between mitochondria and cytosol confirms earlier studies with isolated hepatocytes and perfused liver [refs 1,5,16,17]." For experimental conditions and metabolic states see p.381 table 1 link
Entered by Uri M
ID 111356