Metabolic rates and redox ratios in perfused livers from starved rats

Range Table - link
Organism Rat Rattus norvegicus
Reference Soboll S, Scholz R, Heldt HW. Subcellular metabolite concentrations. Dependence of mitochondrial and cytosolic ATP systems on the metabolic state of perfused rat liver. Eur J Biochem. 1978 Jun 15 87(2):377-90. p.382 table 3PubMed ID668699
Method “From marker enzyme activities and metabolite contents in the unfractionated homogenate and in the different fractions the mitochondrial and cytosolic metabolite contents (nmol/mg protein) were calculated by extrapolating the data to fractions of pure mitochondria and pure cytosol according to Elbers et al. [ref 5]. The subcellular metabolite contents were converted into concentrations assuming a water content of 0.8 and 3.8 µl/mg protein in the mitochondrial and cytosolic compartment, respectively, based on considerations previously discussed by Soboll et al. [ref 1].”
Comments "Under conditions of substrate-free perfusion, the main substrates for energy yielding metabolism are endogenous fatty acids, since high rates of ketogenesis were observed (Table 3, expt H). When suitable substrates are added, such as lactate (Fig. 1) or dihydroxyacetone, the livers synthesize glucose at high rates, whereas ketogenesis is suppressed. Glycolysis is minimal in livers from starved rats. Production of lactate + pyruvate from exogenous glucose is observed at considerable rates only when glucose is present in high concentrations (Table 3, expt H-G)." For experimental conditions and metabolic states see p.381 table 1 link
Entered by Uri M
ID 111355