Total cellular [NAD+]/[NADH]

Range ~2.3 unitless
Organism Mouse Mus musculus
Reference Valsecchi F et al., Metabolic consequences of NDUFS4 gene deletion in immortalized mouse embryonic fibroblasts. Biochim Biophys Acta. 2012 Oct1817(10):1925-36. doi: 10.1016/j.bbabio.2012.03.006. p.1932 fig.4CPubMed ID22430089
Method "Quantification of mitochondrial NAD(P)H autofluorescence." Value extracted visually from fig.4C.
Comments "The ratio between the oxidized and reduced forms of nicotinamide adenine dinucleotide ([NAD+]/[NADH]) is an important component of the cellular redox state. In KO [knockout] cells, this ratio was ~2-fold lower (i.e. more reduced) than in wt cells (Fig. 4C). In contrast, the ratio between [NADP+] and [NADPH] was not affected in KO cells. These findings suggest that CI- [complex I or NADH:ubiquinone oxidoreductase-] mediated NADH oxidation in KO cells is less efficient than in wt cells, inducing a reduction in [NAD+]/[NADH] ratio."
Entered by Uri M
ID 108105