Mitochondrial matrix NADH/NAD ratio (0.009 value for HeLa cell)

Value 0.009 unitless Range: indirectly estimated 0.006 - 0.125 unitless
Organism Unspecified
Reference Chen WW, Freinkman E, Wang T, Birsoy K, Sabatini DM. Absolute Quantification of Matrix Metabolites Reveals the Dynamics of Mitochondrial Metabolism. Cell. 2016 Aug 25 166(5):1324-1337.e11. doi: 10.1016/j.cell.2016.07.040. p.1328 right column top paragraphPubMed ID27565352
Primary Source M. Erecińska, D.F. Wilson, K. Nishiki, Homeostatic regulation of cellular energy metabolism: experimental characterization in vivo and fit to a model Am. J. Physiol., 234 (1978), pp. C82–C89 AND K. Nishiki, M. Erecińska, D.F. Wilson, Energy relationships between cytosolic metabolism and mitochondrial respiration in rat heart, Am. J. Physiol., 234 (1978), pp. C73–C81PubMed ID204196, 204195
Method P.1325 left column top paragraph: "...[investigators] developed a new method that combines rapid immunocapture of epitope-tagged mitochondria with metabolite profiling by liquid chromatography and mass spectrometry (LC/MS)."
Comments P.1328 right column top paragraph: "With regard to the accuracy of [investigators'] measurements, [their] quantification of the matrix NADH/NAD ratio (0.009) agrees with prior studies that indirectly estimated matrix NADH/NAD ratios to span 0.006–0.125, depending on the cell type examined (primary sources). Taken together, these data indicate that [their] LC/MS-based workflow can be used to quantitatively study the metabolic landscape of the mitochondrial matrix." 1st primary source studied various cells. 2nd primary source studied rat heart
Entered by Uri M
ID 112848