||Spinach Spinacia oleracea
||Heineke D, Riens B, Grosse H, Hoferichter P, Peter U, Flügge UI, Heldt HW. Redox Transfer across the Inner Chloroplast Envelope Membrane. Plant Physiol. 1991 Apr95(4):p. 1136 left column 2nd paragraphPubMed ID16668101
||Hydroponic growth. Spinach plants were grown in a climatized chamber with a 9 h light/15 h dark cycle and leaves were stopped in liquid N2 after 8.5 h light or 2 h dark. The frozen tissue was ground and lyophilized. The dry material was homogenized by sonication in a mixture of carbon tetrachloride and heptane, and fractionated by density gradient centrifugation.
||On the order of 0.001. According to the equation: NADH/NAD= ((Glu) * (malate) * K, * K2)/ ((2-OG) * (Asp)*(H+) ) K, = 6.61 (GOT) and K2 = 2.78. 10-12 (MDH) From the data of Table IV and assuming a pH of 7.2, cytosolic NADH to NAD ratio in the light is calculated as 0.0005