Time range for nuclear phase (transcription, transport and export) of gene expression
| Range | 5-40 min |
|---|---|
| Organism | Human Homo sapiens |
| Reference | Mor A, Suliman S, Ben-Yishay R, Yunger S, Brody Y, Shav-Tal Y. Dynamics of single mRNP nucleocytoplasmic transport and export through the nuclear pore in living cells. Nat Cell Biol. 2010 Jun12(6):543-52 p. 546 right column of table 1, Table - link PubMed ID20453848 |
| Method | Researchers established a mammalian cell system harbouring stably integrated genes that enabled the tracking of single mRNPs from the time of transcription and during nuclear transport and export. Gene constructs containing different forms of the human dystrophin (Dys) cDNA fused to a fluorescent protein (green fluorescent protein (GFP) or Cerulean (Cer)) were generated. Active transcription sites and single mRNPs were detected by transient transfection with YFP–MS2-NLS (nuclear localization sequence). |
| Comments | Human U2OS (osteosarcoma) cells. Single-cell analysis shows that the kinetics of the nuclear phase of gene expression (transcription, transport and export) entails a 5–40 min time frame. See BNID 105622 |
| Entered by | Uri M |
| ID | 105650 |