Transcription elongation rate of most genes in HeLa cell

Range ~3.5 (2 - 6) Kb/min
Organism Human Homo sapiens
Reference Fuchs G, Voichek Y, Benjamin S, Gilad S, Amit I, Oren M. 4sUDRB-seq: measuring genomewide transcriptional elongation rates and initiation frequencies within cells. Genome Biol. 2014 May 9 15(5):R69. doi: 10.1186/gb-2014-15-5-r69. p.4 right column bottom paragraphPubMed ID24887486
Method P.7 left column 2nd paragraph: "[Researchers] report here that, by combining the reversible inhibitor DRB [5,6-dichlorobenzimidazole 1-ß-d-ribofuranoside] and 4sU [4-thiouridine] tagging, transcription elongation rates and relative initiation frequencies within intact cells can be relatively easily measured on a genomewide scale...Moreover, by coupling 4sUDRB-seq with depletion of specific transcription-regulatory factors, one should be able to discriminate between factors that impact gene expression by modulating transcription initiation frequencies and those that affect the elongation step."
Comments P.4 right column bottom paragraph: "Overall, [researchers] could measure with high confidence the elongation rates of 1,577 genes. The full list of elongation rates generated by this analysis is presented in Additional file 4. Examples of elongation rate measurements for several representative genes are shown in Figure 3B. As also seen in Figure 3C, while most genes are transcribed at a rate of approximately 3.5 Kb/min, actual transcription elongation rates vary between 2 Kb/min and 6 Kb/min. Overall, these rates are in the same range as those determined previously for a small number of specific genes [ref 28 PMID 19888309, ref 30 PMID 19820712]."
Entered by Uri M
ID 111027