Diffusion coefficients for various mRNAs in the nucleus and the cytoplasm

Range Table - link
Organism Human Homo sapiens
Reference Mor A, Suliman S, Ben-Yishay R, Yunger S, Brody Y, Shav-Tal Y. Dynamics of single mRNP nucleocytoplasmic transport and export through the nuclear pore in living cells. Nat Cell Biol. 2010 Jun12(6):543-52 p. 546 table 1PubMed ID20453848
Method To characterize in vivo export kinetics at the single mRNP (mRNA-protein complex) level, and to understand the relative temporal contribution of export to the total kinetics of nucleocytoplasmic translocation, researchers established a mammalian cell system harbouring stably integrated genes that enabled the tracking of single mRNPs from the time of transcription and during nuclear transport and export. Gene constructs containing different forms of the human dystrophin (Dys) cDNA fused to a fluorescent protein (green fluorescent protein (GFP) or Cerulean (Cer)) were generated. Active transcription sites and single mRNPs were detected by transient transfection with YFP–MS2-NLS (nuclear localization sequence).
Comments Human U2OS (osteosarcoma) cells
Entered by Uri M
ID 105649