Time to transport apolipoprotein B through the cell and export it

Value 30 min
Organism Human Homo sapiens
Reference Olofsson SO, Structure and biosynthesis of apolipoprotein B. Am Heart J. 1987 Feb113(2 Pt 2):446-52.PubMed ID3812204
Primary Source K Bostrom et al, Pulse-chase studies of the synthesis and intracellular transport of apolipoprotein B-100 in Hep G2 cells,J. Biol. Chem. 1986 261: 13800-13806
Method Pulse-chase methodology in the Hep-G2 cell (a human liver carcinoma cell line)
Comments The protein was transported through the cell and secreted within 30 minutes, without undergoing any major change in molecular mass, indicating that it is not synthesized as subunits that are later assembled, or cleaved into smaller components after synthesis.
Entered by Uri M
ID 104600