splicing 0.8-1.6min: transport 2.5-4.4min min
||Human Homo sapiens
||Audibert A, Weil D, Dautry F. In vivo kinetics of mRNA splicing and transport in mammalian cells. Mol Cell Biol. 2002 Oct22(19):6706-18. doi: 10.1128/MCB.22.19.6706-6718.2002 p.6712 right column 3rd paragraph, p.6713 right column bottom paragraph & p.6714 left column 2nd paragraphPubMed ID12215528
||P.6714 left column top paragraph:"In this study [investigators] present a strategy to analyze the splicing and transport rates of pre-mRNA in living cells that relies on the use of regulated promoters. This approach associates the kinetic dimension of transcription regulation by tetracycline with the sensitivity of the RNase protection assay."
||p.6713 right column bottom paragraph:"In conclusion, [investigators] have tested two factors which might have affected the rates of mRNA maturation: cell density and actin metabolism. Neither of these affected conclusively the splicing or the transport rate of β-globin mRNA. Overall, the measure was very robust, with half-lives ranging between 0.8 and 1.6 min for splicing and between 2.5 and 4.4 min for transport." p.6714 left column 2nd paragraph:"Analysis of mRNA export to the cytoplasm.While the biochemistry of mRNA export has been the focus of many recent studies, little is known of its kinetics. In this study, [investigators] provide the first kinetic analysis of mRNA export in mammalian cells. During the first hours of the transcriptional induction of β-globin, the appearance of mRNA in the cytoplasm can be described by a first-order reaction acting on the nuclear population of mRNA. In five independent experiments the corresponding nuclear residence half-lives ranged between 2.5 and 4.4 min, indicating a rapid but not instantaneous transport of mature mRNA to the cytoplasm. As expected, this is much shorter than what has been observed following microinjection of mRNA into the nuclei of Xenopus oocytes [BNID 112186]. It falls, however, within a factor of 2 of the results obtained for the export of specific mRNA from isolated nuclei (ref 39)."