| Value |
3.79
kb/min
Range: ±0.26 Table - link kb/min
|
| Organism |
Human Homo sapiens |
| Reference |
Singh J, Padgett RA. Rates of in situ transcription and splicing in large human genes. Nat Struct Mol Biol. 2009 Nov16(11):1128-33 p.1130 table 1PubMed ID19820712
|
| Method |
P.1129 left column 2nd paragraph: "[Researchers used properties] of DRB [5,6-dichlorobenzimidazole 1-beta-D-ribofuranoside] to develop a method to reversibly
block gene transcription in cultured human cells. [They] incubated the
cells for various times with DRB, [they] then prepared total RNA from the
cultures and assayed for the levels of unspliced pre-mRNA for several
genes. During the time of DRB treatment, most of the unspliced premRNA
should be processed to mature mRNA by the splicing machinery
or degraded (Supplementary Fig. 1). Removal of DRB should then
lead to release of RNAPII from promoter-proximal regions, starting
fresh rounds of transcription. These newly started primary transcripts
will be detectable owing to the presence of introns." |
| Comments |
P.1130 left column 3rd paragraph: "Elongation rates seem to be similar for most genes: Having shown that the DRB release method allows us to follow the progress of RNAPII through chromatin, [researchers] investigated the rate of transcriptional elongation in several large human genes. The selected genes are listed in Table 1, and the data for four of these are shown in Figure 2, along with their exon-intron structures. Several results are notable. First, as shown in Table 1, all gene regions seemed to be transcribed with a markedly uniform rate of about 3.8 kb min^-1. This value is close to the maximum elongation rate (4.3 kb min^-1) determined by Darzacq et al. (ref 17). This suggests that, within genes in their natural chromosomal locations and chromatin states, transcription elongation can be very efficient." This rate is equal to a range of 59-68b/sec. Cells used were a derivative of the neuroblastoma cell line SH-EP Tet-21 and HT1080 cells. See BNID 100662, 101904 |
| Entered by |
Uri M |
| ID |
105566 |