Transcription elongation rates and their confidence intervals in HeLa cells

Range Excel Table - link Kb/min
Organism Human Homo sapiens
Reference Fuchs G, Voichek Y, Benjamin S, Gilad S, Amit I, Oren M. 4sUDRB-seq: measuring genomewide transcriptional elongation rates and initiation frequencies within cells. Genome Biol. 2014 May 9 15(5):R69. doi: 10.1186/gb-2014-15-5-r69. Additional file 4PubMed ID24887486
Method "[Researchers] report here that, by combining the reversible inhibitor DRB [5,6-dichlorobenzimidazole 1-ß-d-ribofuranoside] and 4sU [4-thiouridine] tagging, transcription elongation rates and relative initiation frequencies within intact cells can be relatively easily measured on a genomewide scale...Moreover, by coupling 4sUDRB-seq with depletion of specific transcription-regulatory factors, one should be able to discriminate between factors that impact gene expression by modulating transcription initiation frequencies and those that affect the elongation step."
Comments "Overall, [researchers] could measure with high confidence the elongation rates of 1,577 genes. The full list of elongation rates generated by this analysis is presented in Additional file 4. Examples of elongation rate measurements for several representative genes are shown in Figure 3B. As also seen in Figure 3C, while most genes are transcribed at a rate of approximately 3.5 Kb/min, actual transcription elongation rates vary between 2 Kb/min and 6 Kb/min. Overall, these rates are in the same range as those determined previously for a small number of specific genes [PMID 19888309, PMID 19820712]." See column shaded yellow
Entered by Uri M
ID 111028