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Time required to splice introns

Range	5-10 Table - link min
Organism	Human Homo sapiens
Reference	Singh J, Padgett RA. Rates of in situ transcription and splicing in large human genes. Nat Struct Mol Biol. 2009 Nov16(11):1128-33 p.1131 table 2 and p. 1132 left column bottom paragraphPubMed ID19820712
Method	"[Researchers used properties] of DRB to develop a method to reversibly block gene transcription in cultured human cells. [They] incubated the cells for various times with DRB. [They] then prepared total RNA from the cultures and assayed for the levels of unspliced pre-mRNA for several genes."
Comments	"An unexpected finding of [researchers'] study is that splicing occurred at a similar rate for several U2-dependent introns spanning a wide range of sizes. [They] found lag times for splicing of 5–10 min for introns ranging from 1.2 kb to 240 kb. These times represent the time, after synthesis of the downstream exon, for the appearance of the spliced exons. The splicing rates for small introns agree well with previous measurements (refs 19, 20)." Note-the following 2 sources-[1] Huranova et al., 2010 PMID 20921136 p.83 left column 3rd paragraph (BNID 108452) and [2] Hnilicová et al., 2011 PMID 21818411 p.183 left column 2nd paragraph give a much faster value of 30sec.
Entered by	Uri M
ID	105568