| Range |
Table - link
|
| Organism |
Human Homo sapiens |
| Reference |
Singh J, Padgett RA. Rates of in situ transcription and splicing in large human genes. Nat Struct Mol Biol. 2009 Nov16(11):1128-33 table 2 p.1131 left columnPubMed ID19820712
|
| Method |
Researchers used properties of DRB to develop a method to reversibly
block gene transcription in cultured human cells. They incubated the
cells for various times with DRB, they then prepared total RNA from the
cultures and assayed for the levels of unspliced pre-mRNA for several
genes. During the time of DRB treatment, most of the unspliced premRNA
should be processed to mature mRNA by the splicing machinery
or degraded (Supplementary Fig. 1). Removal of DRB should then
lead to release of RNAPII from promoter-proximal regions, starting
fresh rounds of transcription. These newly started primary transcripts
will be detectable owing to the presence of introns. |
| Comments |
An unexpected finding of researchers' study is that splicing occurred at a similar
rate for several U2-dependent introns spanning a wide range of
sizes. They found lag times for splicing of 5–10 min for introns ranging
from 1.2 kb to 240 kb. These times represent the time, after
synthesis of the downstream exon, for the appearance of the spliced
exons. The splicing rates for small introns agree well with previous
measurements (refs 19, 20 in article). |
| Entered by |
Uri M |
| ID |
105567 |