Time required to splice introns of different sizes and types

Range Table - link
Organism Human Homo sapiens
Reference Singh J, Padgett RA. Rates of in situ transcription and splicing in large human genes. Nat Struct Mol Biol. 2009 Nov16(11):1128-33 table 2 p.1131 left columnPubMed ID19820712
Method Researchers used properties of DRB to develop a method to reversibly block gene transcription in cultured human cells. They incubated the cells for various times with DRB, they then prepared total RNA from the cultures and assayed for the levels of unspliced pre-mRNA for several genes. During the time of DRB treatment, most of the unspliced premRNA should be processed to mature mRNA by the splicing machinery or degraded (Supplementary Fig. 1). Removal of DRB should then lead to release of RNAPII from promoter-proximal regions, starting fresh rounds of transcription. These newly started primary transcripts will be detectable owing to the presence of introns.
Comments An unexpected finding of researchers' study is that splicing occurred at a similar rate for several U2-dependent introns spanning a wide range of sizes. They found lag times for splicing of 5–10 min for introns ranging from 1.2 kb to 240 kb. These times represent the time, after synthesis of the downstream exon, for the appearance of the spliced exons. The splicing rates for small introns agree well with previous measurements (refs 19, 20 in article).
Entered by Uri M
ID 105567