| Comments |
"Having established that DRB/GRO-seq can be used to characterize
transcript elongation in vivo, [researchers] now compared wildtype
cells with cells lacking RECQL5 (Figure 4). Significant
differences were difficult to detect at the early time points. After
40 min, however, the RNAPII-activity wave-shape was clearly
altered in the RECQL5 knockdown cells, with relative depletion
of polymerases in the area up to around 40 kb and with a corresponding
density increase in the region from 40 kb to the transcription
wave front at ~100 kb (Figure 4A), indicating that
RNAPII generally transcribed further into genes in the absence
of RECQL5. Figure 4B shows a specific example in which
more nascent RNAPII transcript reads were detected 80–120
kb into the CTNNNBL1 gene in the absence of RECQL5 than in
normal cells (more examples in Figure S2A)...elongation rates were significantly higher in cells
lacking RECQL5: the median elongation rate in the 25–40 min
interval was 3.96 kb/min in RECQL5-depleted cells and
3.13 kb/min in wild-type cells, a rate increase of 27% in the
absence of RECQL5 (p value 1.22×10^-8) (Figure 4D)." |