RNA Pol II elongation rate in HEK 293 cells

Value 3.13 kb/min
Organism Human Homo sapiens
Reference Saponaro et al., RECQL5 controls transcript elongation and suppresses genome instability associated with transcription stress. Cell. 2014 May 22 157(5):1037-49. doi: 10.1016/j.cell.2014.03.048. p.1042 left columnPubMed ID24836610
Comments "Having established that DRB/GRO-seq can be used to characterize transcript elongation in vivo, [researchers] now compared wildtype cells with cells lacking RECQL5 (Figure 4). Significant differences were difficult to detect at the early time points. After 40 min, however, the RNAPII-activity wave-shape was clearly altered in the RECQL5 knockdown cells, with relative depletion of polymerases in the area up to around 40 kb and with a corresponding density increase in the region from 40 kb to the transcription wave front at ~100 kb (Figure 4A), indicating that RNAPII generally transcribed further into genes in the absence of RECQL5. Figure 4B shows a specific example in which more nascent RNAPII transcript reads were detected 80–120 kb into the CTNNNBL1 gene in the absence of RECQL5 than in normal cells (more examples in Figure S2A)...elongation rates were significantly higher in cells lacking RECQL5: the median elongation rate in the 25–40 min interval was 3.96 kb/min in RECQL5-depleted cells and 3.13 kb/min in wild-type cells, a rate increase of 27% in the absence of RECQL5 (p value 1.22×10^-8) (Figure 4D)."
Entered by Uri M
ID 111212