Decrease in velocity of the reaction when isocitrate replaces homoisocitrate as substrate
| Value | 216 Fold |
|---|---|
| Organism | Budding yeast Saccharomyces cerevisiae |
| Reference | Lin Y, Alguindigue SS, Volkman J, Nicholas KM, West AH, Cook PF. Complete kinetic mechanism of homoisocitrate dehydrogenase from Saccharomyces cerevisiae. Biochemistry. 2007 Jan 2346(3):890-8.PubMed ID17223711 |
| Method | Determination of the Mg-HIc Dissociation Constant by 1H NMR |
| Comments | In contrast to HIc, the uncomplexed form of isocitrate and Mg2+ bind to the enzyme, unlike the situation with MgHIc. |
| Entered by | Uri M |
| ID | 102568 |