GSH [glutathione] half-life under standard conditions

Range ~90 min
Organism Budding yeast Saccharomyces cerevisiae
Reference Baudouin-Cornu P, Lagniel G, Kumar C, Huang ME, Labarre J. Glutathione degradation is a key determinant of glutathione homeostasis. J Biol Chem. 2012 Feb 10 287(7):4552-61. doi: 10.1074/jbc.M111.315705 abstract, p.4555 left column top paragraph & supplementary information p.15PubMed ID22170048
Method Abstract: "Combining mathematical models and (35)S labeling, [investigators] analyzed Saccharomyces cerevisiae sulfur metabolism. This led [them] to the observation that GSH [glutathione] recycling is markedly faster than previously estimated. [They] set up additional in vivo assays and concluded that under standard conditions, GSH half-life is around 90 min."
Comments P.4554 right column bottom paragraph: "Thus, [investigators'] model strongly suggests that the time required for converting half the concentration of total GSH into Cys ([cor]T1/2[GSH]) is around 90 min (supplemental information, Part1). Determination of GSH Half-lives—The half-life of a molecule is usually defined as the time required to degrade 50% of the initial amount of this molecule (e.g. [cor]T1/2[GSH] for GSH, see above). For practical reasons, [they] also use in this work the notion of apparent half-life of GSH ([app]T1/2 [GSH]), which [they] define as the time required to incorporate into proteins 50% of the sulfur atoms of the initial pool of GSH."
Entered by Uri M
ID 113864