Lipid molecule diffusion constant on L-6 myoblast membranes

Value 0.9 µm^2/sec
Organism Rat Rattus norvegicus
Reference Schlessinger J. et al., Lateral transport of a lipid probe and labeled proteins on a cell membrane. Science. 1977 Jan 21 195(4275):307-9. p.307 middle column bottom paragraphPubMed ID556653
Method P.307 middle column bottom paragraph: "[Investigators] have used 3,3-dioctadecylindocarbocyanine iodide (diI) dissolved in the lipid portion of the plasma membrane as a fluorescent analog to indicate lipid behavior. Incorporation of the diI in the plasma membrane of these cells is indicated by detailed studies of peripheral fluorescence of various labeled cells (ref 11 link ). The diI does mimic the diffusion of phospholipids in model lipid bilayers (ref 12) but may differ from natural lipids in its interaction with membrane proteins. Figure 1a presents a typical FPR [fluorescence photobleaching recovery] recovery curve for diI. It coincides with theory (ref 8) for diffusion with a single coefficient D=9.2x10^-9cm^2/sec. The mean value (± the standard deviation) obtained from measurements on 18 cells is D=(9.0±4.0)x10^-9cm^2/sec. This result was confirmed by the value D=(8.0±3.0)x10^-9cm^2/sec obtained by a different method, namely, fluorescence correlation spectroscopy (ref 13)."
Comments Gartner et al., 1975 PMID 1201086 p.972 2nd paragraph: "L6 is a permanent cell line derived from rat skeletal muscle (ref 1). L6 is particularly interesting because it differentiates in-vitro. Myoblasts proliferate and are the undifferentiated form of L6."
Entered by Uri M
ID 112471