Measurements by FPR [fluorescence photobleaching recovery] at three different positions on a single cell surface labeled with TNBS and rhodamine-marked antibodies to DNP

Range Table - link
Organism Rat Rattus norvegicus
Reference Schlessinger J. et al., Lateral transport of a lipid probe and labeled proteins on a cell membrane. Science. 1977 Jan 21 195(4275):307-9. p.308 table 1PubMed ID556653
Comments P.308 left column 2nd paragraph: "To test the generality of these results and discover whether FITC [fluorescein isothiocyanate] was selectively labeling proteins with exceptional diffusion coefficients, [investigators] have applied a distinctly different labeling procedure. Plasma membrane components of L-6 were conjugated with 2,4,6-trinitrobenzene sulfonate (TNBS) and marked with rhodamine-labeled antibody to dinitrophenol (DNP) (refs 16, 17). Figure 1b presents a recovery curve (typical of 15 cells) from a cell labeled in this way: D=1.9x10^-10cm^2/sec, with a fluorescence recovery of 54 percent. In general, results obtained with TNBS-labeled components closely parallel those obtained with FITC. Hence [investigators’] results seem to reflect the behavior of a representative collection of membrane components (expected to be mostly proteins) able to bind FITC or TNBS (for example, with exposed lysine amine groups). The components labeled with TNBS and then antibody to DNP components also show substantial variation of D and fractional recovery at different positions in the same cell (Table 1)." Gartner et al., 1975 PMID 1201086 p.972 2nd paragraph: "L6 is a permanent cell line derived from rat skeletal muscle (ref 1). L6 is particularly interesting because it differentiates in-vitro. Myoblasts proliferate and are the undifferentiated form of L6." TNBS=2,4,6-trinitrobenzene sulfonate. DNP=dinitrophenol. See notes beneath table
Entered by Uri M
ID 112472