Apparent diffusion coefficient of NLS-EGFP in the cortical cytoplasm of the embryo

Value 24 μm^2/sec Range: ±1 μm^2/sec
Organism Fruit fly Drosophila melanogaster
Reference Abu-Arish A, Porcher A, Czerwonka A, Dostatni N, Fradin C. High mobility of bicoid captured by fluorescence correlation spectroscopy: implication for the rapid establishment of its gradient. Biophys J. 2010 Aug 9 99(4):L33-5. doi: 10.1016/j.bpj.2010.05.031. p.L34 right column 2nd paragraphPubMed ID20712981
Method Fluorescence correlation spectroscopy (FCS) "To understand how the Bcd concentration gradient could be established so quickly, [researchers] obtained the mobility of Bcd- EGFP in the anterior cortical cytoplasm of the embryos using single-point FCS and [researchers] compared it to the mobility of a control NLS-EGFP fusion protein."
Comments "The data did not allow clear discrimination between more complex diffusion models, yet all models agreed that the mobility of Bcd-EGFP in the cortical cytoplasm of the embryos corresponds to an average diffusion coefficient lying between 5 and 10 µm^2/s, with a likely value D = 7.4 ± 0.4 µm^2/s. The mobility of the control protein NLS-EGFP [Nuclear localization signal-EGFP] was approximately threefold larger [than Bicoid-EGFP], with an apparent diffusion coefficient D=24±1 µm^2/s."
Entered by Uri M
ID 109209