Diffusion coefficient of oligo(dT) (43-mers) in nucleus of cultured rat myoblasts

Value 40 µm^2/sec
Organism Rat Rattus norvegicus
Reference Lukacs GL, Haggie P, Seksek O, Lechardeur D, Freedman N, Verkman AS. Size-dependent DNA mobility in cytoplasm and nucleus. J Biol Chem. 2000 Jan 21 275(3):1625-9. p.1628 right column 2nd paragraphPubMed ID10636854
Primary Source Politz JC, Browne ES, Wolf DE, Pederson T. Intranuclear diffusion and hybridization state of oligonucleotides measured by fluorescence correlation spectroscopy in living cells. Proc Natl Acad Sci U S A. 1998 May 26 95(11):6043-8.PubMed ID9600914
Method (Primary source:) Fluorescence correlation spectroscopy
Comments Although the majority of oligo(dT) was immobile, probably because of hybridization to poly(A) sequences, a significant fraction of the poly(dT) was free with an apparent diffusion coefficient of 4^10^-7 cm2/s, nearly the same as that measured in aqueous solutions.
Entered by Uri M
ID 106084