Half-lives of specific mRNAs

Range 3 to >90 min
Organism Budding yeast Saccharomyces cerevisiae
Reference Bregman A, Avraham-Kelbert M, Barkai O, Duek L, Guterman A, Choder M. Promoter elements regulate cytoplasmic mRNA decay. Cell. 2011 Dec 23 147(7):1473-83. doi: 10.1016/j.cell.2011.12.005. p.1473 right column 2nd paragraphPubMed ID22196725
Primary Source Wang, Y., Liu, C.L., Storey, J.D., Tibshirani, R.J., Herschlag, D., and Brown, P.O. (2002). Precision and functional specificity in mRNA decay. Proc. Natl. Acad. Sci. USA 99, 5860–5865.PubMed ID11972065
Method (primary source abstract:) "By using DNA microarrays, [researchers] precisely measured the decay of each yeast mRNA, after thermal inactivation of a temperature-sensitive RNA polymerase II."
Comments "Two major cytoplasmic decay pathways exist. Both are initiated by shortening of the mRNA poly(A) tail. The mRNA can then be exonucleolytically degraded by the exosome from 3' to 5' or by the Xrn1p exonuclease from 5' to 3'. The latter pathway involves removal of the mRNA 5' cap (m(7)GpppN) (Decker and Parker, 1993), which is a prerequisite stage for Xrn1p activity (Coller and Parker, 2004 Larimer et al., 1992 Parker and Song, 2004). Although most yeast mRNAs are degraded by either or both of these pathways, the half-lives of specific mRNAs vary widely, ranging from 3 min to more than 90 min (primary source)."
Entered by Uri M
ID 110027