Half life of mRNA of 20S proteasome core protein subunit

Value 13 min Range: ±3 min
Organism Budding yeast Saccharomyces cerevisiae
Reference Wang Y, Liu CL, Storey JD, Tibshirani RJ, Herschlag D, Brown PO. Precision and functional specificity in mRNA decay. Proc Natl Acad Sci U S A. 2002 Apr 30 99(9):5860-5 p.5862 right column 3rd paragraphPubMed ID11972065
Method By using DNA microarrays, researchers precisely measured the decay of each yeast mRNA, after thermal inactivation of a temperature-sensitive RNA polymerase II.
Comments The half-lives of the 4,687 mRNAs analyzed varied widely, ranging from ~3 min to more than 90 min, with a mean of 23 min and median of 20 min. The 20S proteasome core is a stoichiometric complex of 14 different protein subunits (ref 30). We were able to obtain good measurements of the decay of 13 of the 14 corresponding mRNAs all decayed at closely matched rates, with t1/2 = 13 ± 3 min
Entered by Uri M
ID 104744