Rate of overall mutations (including non deleterious)

Value 0.32 mutation/(genome*cell division)
Organism Budding yeast Saccharomyces cerevisiae
Reference Lynch M, Sung W, Morris K, Coffey N, Landry CR, Dopman EB, Dickinson WJ, Okamoto K, Kulkarni S, Hartl DL, Thomas WK. A genome-wide view of the spectrum of spontaneous mutations in yeast. Proc Natl Acad Sci U S A. 2008 Jul 8105(27):9272-7. Table 3 Table - link PubMed ID18583475
Primary Source 1. Baer CF, 2. et al. (2006) Cumulative effects of spontaneous mutations for fitness in Caenorhabditis: Role of genotype, environment and stress. Genetics 174:1387–1395.PubMed ID16888328
Method Analyses are based on an examination of parallel MA lines of a key model system, the yeast Saccharomyces cerevisiae. The initially isogenic lines were passed through 200 single-cell bottlenecks on a 3- to 4-day cycle of clonal growth for a total of ˜4,800 cell divisions per line. Spontaneous mutations were accumulated in 32 independent sets of haploid lines, 8 in each of four series, A–D, all initiated from the same clonal parent derived from a single laboratory stock, FY10 (leu2?1, ura3–52, MATa)
Comments 0.0041 base substitutions, 0.0002 small insertion/deletions in complex sequence, 0.0019 microsatellite mutations, and 0.3094 homopolymer mutations.
Entered by Uri M
ID 103521