Median half-life of mRNA transcripts

Value 9 Hours
Organism Mammalian tissue culture cell
Reference Schwanhäusser B, Busse D, Li N, Dittmar G, Schuchhardt J, Wolf J, Chen W, Selbach M. Global quantification of mammalian gene expression control. Nature. 2011 May 19 473(7347):337-42. p.338 left column top paragraphPubMed ID21593866
Method Newly synthesized RNA can be labelled with the nucleoside analogue 4-thiouridine (4sU). 4sU-containing mRNA can be purified and compared with the preexisting fraction to compute mRNA half-lives.
Comments NIH3T3 (mouse fibroblast) cells. See BNID 106377
Entered by Uri M
ID 106378