Rate of protein synthesis from mRNA transcript (median)

Value ~ Proteins/mRNA/hour Range: original 2011 value ~140: updated 2013 value 1,000 Proteins/mRNA/hour
Organism Mammalian tissue culture cell
Reference Schwanhäusser B, Busse D, Li N, Dittmar G, Schuchhardt J, Wolf J, Chen W, Selbach M. Global quantification of mammalian gene expression control. Nature. 2011 May 19 473(7347):337-42. p.339 left column bottom paragraph Corrected value is from Schwanhäusser B et al., Corrigendum: Global quantification of mammalian gene expression control. Nature. 2013 Mar 7 495(7439):126-7. doi: 10.1038/nature11848. p.126 right column bottom paragraphPubMed ID21593866, 23407496
Method 2011 paper, p.337 right column bottom paragraph: "[Researchers] used parallel metabolic pulse labelling with amino acids and 4sU [the nucleoside analogue 4-thiouridine] to measure simultaneously protein and mRNA turnover in a population of exponentially growing non-synchronized NIH3T3 mouse fibroblasts (Fig. 1a). Protein samples were collected at three time points, measured by liquid chromatography and online tandem mass spectrometry (LC-MS/MS) and analysed with the MaxQuant software package (ref 17)."
Comments Original value, 2011 paper, p.339 left column bottom paragraph: "[Investigators] find a median translation rate constant of about 140 proteins per mRNA per hour." Updated value, 2013 paper, p.126 right column top paragraph: "[Investigators] erroneously used the slope and the offset from an unrelated experiment to scale protein levels, resulting in a systematic underestimation of protein levels and derived translation rate constants. [They] apologize for this error and any confusion it may have caused. When the error was corrected, the median levels of detected proteins increased about threefold and the ratio of average protein to messenger RNA increased from 900 to 2,800. The median and apparent maximum translation rate constants increased from 40 to 140 and from 180 to 1,000 proteins per mRNA per hour, respectively. Consequently, the estimated maximum translation rate constant in sea urchin embryos at 15˚C (140 proteins per mRNA per hour) is lower than [their] corrected prediction for mouse fibroblasts (1,000 proteins per mRNA per hour)." NIH3T3 (mouse fibroblast) cells.
Entered by Uri M
ID 106382