Median half lives of mRNA transcripts in cells with generation times of 20 (E.coli), 90 (S.cerevisiae) and 3,000 (H.sapiens) min, respectively

Range 5 min for E. coli: 21 min for S. cerevisiae: 600 min for human HepG2/Bud8 cells Hours^-1
Organism Various
Reference Yang E. et al., Decay rates of human mRNAs: correlation with functional characteristics and sequence attributes. Genome Res. 2003 Aug13(8):1863-72. doi:10.1101/gr.1272403 p.1864 left column bottom paragraphPubMed ID12902380
Primary Source Bernstein JA, Khodursky AB, Lin PH, Lin-Chao S, Cohen SN. Global analysis of mRNA decay and abundance in Escherichia coli at single-gene resolution using two-color fluorescent DNA microarrays. Proc Natl Acad Sci U S A. 2002 Jul 23 99(15):9697-702 AND Wang Y, Liu CL, Storey JD, Tibshirani RJ, Herschlag D, Brown PO. Precision and functional specificity in mRNA decay. Proc Natl Acad Sci U S A. 2002 Apr 30 99(9):5860-5PubMed ID12119387, 11972065
Method (for human cells, reference:) "To study the rates of mRNA degradation (“decay”) in human cells, researchers measured changes in mRNA levels following application of the RNA polymerase inhibitor Actinomycin D with Affymetrix U95Av2 high-density oligonucleotide arrays."
Comments "[Investigators] thus believe that [their] 10-h median half-life figure is accurate to within 10%. Comparing this median half-life with the median half-lives of transcripts in yeast and bacteria, it appears that the half-life of the mRNA pool of a cell scales roughly in proportion to the length of the cell cycle: cell cycle lengths of 20, 90, and 3000 min correspond to median half-lives of 5, 21, and 600 min, respectively, for E. coli, S. cerevisiae, and human HepG2/Bud8 cells (primary sources)."
Entered by Uri M
ID 106869