Average cellular transcription rates predicted by model

Value 2 mRNA/hour
Organism Mammalian tissue culture cell
Reference Schwanhäusser B, Busse D, Li N, Dittmar G, Schuchhardt J, Wolf J, Chen W, Selbach M. Global quantification of mammalian gene expression control. Nature. 2011 May 19 473(7347):337-42. p.339 left column 3rd paragraph and fig.3bPubMed ID21593866
Method Newly synthesized RNA can be labelled with the nucleoside analogue 4-thiouridine (4sU). 4sU-containing mRNA can be purified and compared with the preexisting fraction to compute mRNA half-lives. See model in p.339 fig. 3a
Comments NIH3T3 (mouse fibroblast) cells. Average cellular transcription rates predicted by the model spanned two orders of magnitude with a median of about two mRNA molecules per hour.
Entered by Uri M
ID 106379