Mouse fibroblast proteins and mRNAs - abundances and halflives

Range Excel Table - link
Organism Mammalian tissue culture cell
Reference Schwanhäusser B, Busse D, Li N, Dittmar G, Schuchhardt J, Wolf J, Chen W, Selbach M. Global quantification of mammalian gene expression control. Nature. 2011 May 19 473(7347):337-42. Supplementary table S3PubMed ID21593866
Method Stable isotope labelling by amino acids in cell culture (SILAC). Newly synthesized RNA can be labelled with the nucleoside analogue 4-thiouridine (4sU). 4sU-containing mRNA can be purified and compared with the preexisting fraction to compute mRNA half-lives.
Comments NIH3T3 (mouse fibroblast) cells. See columns N, Q, T, W for protein abundance, mRNA abundance, protein halflives and mRNA halflives, respectively
Entered by Uri M
ID 106386