Diffusion coefficient of nucleoplasmic chromatin in nucleus of HT1080 (human fibrosarcoma) cell

Value 1.3e-4 µm^2/sec
Organism Human Homo sapiens
Reference Görisch SM, Lichter P, Rippe K. Mobility of multi-subunit complexes in the nucleus: accessibility and dynamics of chromatin subcompartments. Histochem Cell Biol. 2005 Mar123(3):217-28.PubMed ID15830242
Primary Source Chubb JR, Boyle S, Perry P, Bickmore WA. Chromatin motion is constrained by association with nuclear compartments in human cells. Curr Biol. 2002 Mar 19,12(6):439-45PubMed ID11909528
Method (Primary source) Generation of lacO Integrant Cell Lines, Fluorescence In Situ Hybridization and Immunofluorescence, Visualization of Chromatin Motion in Live HT-1080 Cells.
Comments HT1080 is a human fibrosarcoma cell line.The movements of different sites in human living cells revealed that loci at nucleoli or the nuclear periphery are significantly less mobile than more nucleoplasmic loci. For the latter sites, a mean diffusion coefficient in human cells of 1.3×10^–4 um^2/sec was reported (Chubb et al. 2002)
Entered by Uri M
ID 104273