Protein molecule diffusion constant on L-6 myoblast membranes

Range ~0.02 µm^2/sec
Organism Rat Rattus norvegicus
Reference Schlessinger J. et al., Lateral transport of a lipid probe and labeled proteins on a cell membrane. Science. 1977 Jan 21 195(4275):307-9. p.308 middle column bottom paragraphPubMed ID556653
Method P.307 left column bottom paragraph: "[Investigators] used fluorescence photobleaching recovery (FPR) (refs 2, 7-9) to measure lateral transport. An intense laser light pulse irreversibly bleaches the fluorophore in a small region of the membrane. Transport coefficients are calculated from rates of recovery of fluorescence in the bleached region due to entry of unbleached fluorophores from adjacent parts of the membrane. In principle, FPR can be used to distinguish among diffusion and mechanisms of systematic driven transport (ref 8)."
Comments P.308 middle column bottom paragraph: "(iii) Proteins on the plasma membrane labeled with FITC [fluorescein isothiocyanate] and TNBS [2,4,6-trinitrobenzene sulfonate] show similar rates, D~2×10^-10 cm^2/sec, and extents of fluorescence recovery." Gartner et al., 1975 PMID 1201086 p.972 2nd paragraph: "L6 is a permanent cell line derived from rat skeletal muscle (ref 1). L6 is particularly interesting because it differentiates in-vitro. Myoblasts proliferate and are the undifferentiated form of L6."
Entered by Uri M
ID 112473