Typical empirical protein concentration in biosynthetic pathways

Value 2480 Copies/cell Range: × /4.7 Copies/cell
Organism Budding yeast Saccharomyces cerevisiae
Reference Liebermeister W, Klipp E. Bringing metabolic networks to life: integration of kinetic, metabolic, and proteomic data. Theor Biol Med Model. 2006 Dec 15 3: 42. Table - link PubMed ID17173670
Method Enzyme concentrations were roughly guessed from protein molecule numbers in the yeast S. cerevisiae, measured in a GFP assay [Huh et al, PMID 14562095]. To convert molecule numbers into concentrations, researchers assumed a spherical cell of radius 6 µm. The protein concentration reads c= Number of molecules/(Number Avogadro ×Vcell) M, with Avogadro's constant NA=6.022×10^23 and the cell volume measured in litres. The range is calculated by dividing and multiplying the geometric mean by the exponent of the standard deviation of the natural logarithm, 4.7. 2480/4.7=528copies/cell. 2480×4.7=11,656 copies/cell.
Entered by Uri M
ID 105248