| Value |
6000
Copies/cell
|
| Organism |
Budding yeast Saccharomyces cerevisiae |
| Reference |
Borggrefe T, Davis R, Bareket-Samish A, Kornberg RD. Quantitation of the RNA polymerase II transcription machinery in yeast. J Biol Chem. 2001 Dec 14 276(50):47150-3 Table - link PubMed ID11591727
|
| Method |
Tandem Affinity Purification (TAP) tags and dot blot analysis have been used to measure the amounts of RNA polymerase II transcription proteins in crude yeast extracts. |
| Comments |
The measurements showed comparable amounts of RNA polymerase II, TFIIE, and TFIIF, lower levels of TBP and TFIIB, and still lower levels of Mediator and TFIIH. |
| Entered by |
Uri M |
| ID |
104517 |