Number of Mediator (Med8) molecules per haploid cell

Value 6000 Copies/cell
Organism Budding yeast Saccharomyces cerevisiae
Reference Borggrefe T, Davis R, Bareket-Samish A, Kornberg RD. Quantitation of the RNA polymerase II transcription machinery in yeast. J Biol Chem. 2001 Dec 14 276(50):47150-3 Table - link PubMed ID11591727
Method Tandem Affinity Purification (TAP) tags and dot blot analysis have been used to measure the amounts of RNA polymerase II transcription proteins in crude yeast extracts.
Comments The measurements showed comparable amounts of RNA polymerase II, TFIIE, and TFIIF, lower levels of TBP and TFIIB, and still lower levels of Mediator and TFIIH.
Entered by Uri M
ID 104517