| Method |
Experimental values for turnover rates, substrate, product, and inhibition constants were taken from the Brenda database [BNID 100598], To obtain energy constants, researchers used Gibbs free energies
of formation predicted from the molecule structures,
using the group contribution method [Mavrovouniotis PMID 18595046], Enzyme concentrations were roughly guessed from protein
molecule numbers in the yeast S. cerevisiae, measured
in a GFP assay [Huh et al, PMID 14562095], Equilibrium constants were taken from the NIST database [BNID 103383], The concentrations of 49 metabolites were taken from
a literature survey [Albe et al PMID 2200929]. Concentrations measured in different
species were averaged as described [Liebermeister PMID 16379536] |