Typical empirical parameter ranges of biosynthetic pathways

Range Table - link
Organism Various
Reference Liebermeister W, Klipp E. Bringing metabolic networks to life: integration of kinetic, metabolic, and proteomic data. Theor Biol Med Model. 2006 Dec 15 3: 42.PubMed ID17173670
Method Experimental values for turnover rates, substrate, product, and inhibition constants were taken from the Brenda database [BNID 100598], To obtain energy constants, researchers used Gibbs free energies of formation predicted from the molecule structures, using the group contribution method [Mavrovouniotis PMID 18595046], Enzyme concentrations were roughly guessed from protein molecule numbers in the yeast S. cerevisiae, measured in a GFP assay [Huh et al, PMID 14562095], Equilibrium constants were taken from the NIST database [BNID 103383], The concentrations of 49 metabolites were taken from a literature survey [Albe et al PMID 2200929]. Concentrations measured in different species were averaged as described [Liebermeister PMID 16379536]
Entered by Uri M
ID 105244