Decrease in diffusion coefficient in cytoplasm compared to dilute solution

Range 10 to 30 Fold
Organism Bacteria Escherichia coli
Reference Pielak GJ, Li C, Miklos AC, Schlesinger AP, Slade KM, Wang GF, Zigoneanu IG. Protein nuclear magnetic resonance under physiological conditions. Biochemistry. 2009 Jan 20 48(2):226-34. p.227 right column 2nd paragraphPubMed ID19113834
Primary Source 20) Mullineaux CW, Nenninger A, Ray N, Robinson C. Diffusion of green fluorescent protein in three cell environments in Escherichia coli. J Bacteriol. 2006 May188(10):3442-8. AND (21) Elowitz et al., Protein mobility in the cytoplasm of Escherichia coli. J Bacteriol. 1999 Jan181(1):197-203. AND (22) Zhou HX, Rivas G, Minton AP. Macromolecular crowding and confinement: biochemical, biophysical, and potential physiological consequences. Annu Rev Biophys. 2008 37 375-97.PubMed ID16672597, 9864330, 18573087
Method Using fluorescence to measure the translational diffusion of GFP, either by itself or fused to other proteins.
Comments Whereas the eukaryotic cytoplasm slows diffusion no more than 4-fold (BNID 106192), the cytoplasm of E. coli slows diffusion 10-30-fold (primary sources). Please note that attachment of GFP to the protein of interest adds 27 kDa of bulk to the target protein, and provides information only about the environment of the fluorophore, which occurs only once in the target protein.
Entered by Uri M
ID 106193