Ratio between GFP diffusion rate in cytoplasm of DH5α strain and in water

Value 0.09 Unitless
Organism Bacteria Escherichia coli
Reference Elowitz MB, Surette MG, Wolf PE, Stock JB, Leibler S. Protein mobility in the cytoplasm of Escherichia coli. J Bacteriol. 1999 Jan181(1):197-203. p.201 right column bottom paragraphPubMed ID9864330
Method FRAP- Fluorescence Recovery After Photobleaching and photoactivation. Value of 0.09 arrived at by dividing Dcytoplasm 7.7±2.5 (BNID 100193) by Dwater 87µm^2/s (BNID 100301)(see comments section)
Comments P.201 right column bottom paragraph: "[Investigators] have performed FRAP and photoactivation experiments to measure the apparent diffusion coefficients of GFP and GFP fusion proteins in living E. coli cells. Differences with previous FRAP experiments used in other systems included the use of red GFP photoactivation, requiring 10 times less energy than photobleaching, and the analysis of recovery data throughout the cell rather than only in the irradiated spot. The apparent diffusion coefficient for GFP in bacterial cytoplasm is 7.7 ± 2.5 μm^2/s, about 11 times lower than in water (87 μm^2/s) (refs 25, 26) and significantly lower than in eukaryotic cells (∼27 μm^2/s) (ref 25) and mitochondria (20 to 30 μm^2/s) (ref 19)." DH5alpha strain
Entered by Uri M
ID 105896