Diffusion coefficient of DNA in water

Range 0.81 to 53 µm^2/s
Organism Generic
Reference Lukacs GL, Haggie P, Seksek O, Lechardeur D, Freedman N, Verkman AS. Size-dependent DNA mobility in cytoplasm and nucleus. J Biol Chem. 2000 Jan 21 275(3):1625-9. abstractPubMed ID10636854
Method Abstract: "[Investigators] have measured the translational diffusion of fluorescein-labeled double-stranded DNA fragments (in base pairs (bp): 21, 100, 250, 500, 1000, 2000, 3000, 6000) after microinjection into cytoplasm and nucleus of HeLa cells. Diffusion was measured by spot photobleaching using a focused argon laser spot (488 nm)."
Comments Abstract: "In aqueous solutions, diffusion coefficients of the DNA fragments in water (Dw) decreased from 53 × 10^−8 to 0.81 × 10^−8 cm^2/s for sizes of 21–6000 bp, Dw was related empirically to DNA size:Dw = 4.9 × 10^−6cm^2/s·[bp size]^−0.72. DNA diffusion coefficients in cytoplasm (D cyto) were lower than Dw and depended strongly on DNA size. Dcyto/Dw decreased from 0.19 for a 100-bp DNA fragment to 0.06 for a 250-bp DNA fragment and was <0.01 for >2000 bp." See BNID 106087
Entered by Uri M
ID 106089