Lower limit of propulsive forces required for nuclear organelles to undergo processive transport in intranuclear region of mouse embryonic tissue cell

Range 3-15 picoNewton
Organism Mammalian tissue culture cell
Reference Tseng Y, Lee JS, Kole TP, Jiang I, Wirtz D. Micro-organization and visco-elasticity of the interphase nucleus revealed by particle nanotracking. J Cell Sci. 2004 Apr 15117(Pt 10):2159-67.PubMed ID15090601
Method Through the use of particle nanotracking, researchers detect and quantify the micro-organization as well as the viscoelastic properties of the intranuclear region in single, live, interphase somatic cells.
Comments The mean shear viscosity and elasticity of the intranuclear region of Swiss 3T3 fibroblasts are 520 Poise (P) and 180 dyn/cm(2), respectively. These measurements determine a lower bound of the propulsive forces (3-15 picoNewton) required for nuclear organelles such as promyelocytic-leukemia bodies to undergo processive transport within the nucleus by overcoming friction forces set by the intranuclear viscosity.
Entered by Uri M
ID 104281